HL-sixty cells were incubated with MMRI-dos0-PE or isotype manage-PE (10?g/ml) towards the ice to own 20 minute
HL-sixty cells were incubated with MMRI-dos0-PE or isotype manage-PE (10?g/ml) towards the ice to own 20 minute Antibody coated cells were then incubated at 37°C/5% CO2 for the indicated times to allow internalisation. After incubation, a secondary GAM IgG-AF488 antibody was applied to the samples for 20 min on ice to detect remaining surface antibody […]